Abstract
Chalk brood in honey bees was found in Japan. The causative fungus, Ascosphaera apis, was isolated from the dead and discolored larvae and pupae of honey bees collected from Gifu in 1978. This finding was the first in Japan. In mycological studies, colonies on MY 20 agar were spreading broadly with deeply floccose, surface hyaline to dark gray. The odor was conspicuously sour. Spore cysts were produced in a fairly dense layer. According to microscopic observations, spore cysts were globose, 75-100μm in diam., black to dark brown and glabrous. Spore balls were 11-17μm and spherical to subglobose. Ascospores were hyaline, one-celled, smooth, ellipsoidal and 3.0-4.0×1.0-2.5μm. Conidial structure was not produced. With natural occurrence of chalk brood, the problem of prevention for the fungus, A. apis, was pointed out.