牛項靭帯,大動脈および肺を素材としたピリジニウム環含有架橋アミノ酸の分離調製

中村 文彦; 須山 享三

doi:10.2508/chikusan.62.186

中村文彦, 須山享三

著者情報

ジャーナルフリー

1991 年 62 巻 2 号 p. 186-193

DOI https://doi.org/10.2508/chikusan.62.186

詳細

発行日: 1991/02/25 受付日: 1990/07/13 J-STAGE公開日: 2008/03/10 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : A rapid large scale procedure was devised for the purpose of purifying crosslinking amino acids containing quaternary pyridinium skeleton; desmosine, isodesmosine, neodesmosine and allodesmosine, from elastin of bovine ligamentum nuchae, aorta and lung.
The first step fractionation of crosslinking amino acids from elastin hydrolysate was carried out by means of activated charcoal column. Hydrophobic amino acids (tyrosine and phenylalanine) which could not be removed from crosslinking amino acids by an activated charcoal column were removed by the use of a silica gel normal phase column. The resolution of desmosine and isodesmosine was achieved on a preparative silica gel high performance liquid chromatographic (HPLC) column using n-propanol/water/ 25% ammonia as a solvent. Both isomers were obtained in more than 65% yields.
Cellulose column chromatography and normal phase HPLC using a solvent system of ethyl acetate/acetic acid/water made possible the preparation of neodesmosine. Allodesmosine was separated by means of a normal phase HPLC using ethyl acetate/acetic acid/water and n-propanol/water/25% ammonia.
Right : A rapid large scale procedure was devised for the purpose of purifying crosslinking amino acids containing quaternary pyridinium skeleton; desmosine, isodesmosine, neodesmosine and allodesmosine, from elastin of bovine ligamentum nuchae, aorta and lung.
The first step fractionation of crosslinking amino acids from elastin hydrolysate was carried out by means of activated charcoal column. Hydrophobic amino acids (tyrosine and phenylalanine) which could not be removed from crosslinking amino acids by an activated charcoal column were removed by the use of a silica gel normal phase column.
The resolution of desmosine and isodesmosine was achieved on a preparative silica gel high performance liquid chromatographic (HPLC) column using n-propanol/water/ 25% ammonia as a solvent. Both isomers were obtained in more than 65% yields.Cellulose column chromatography and normal phase HPLC using a solvent system of ethyl acetate/acetic acid/water made possible the preparation of neodesmosine. Allodesmosine was separated by means of a normal phase HPLC using ethyl acetate/acetic acid/water and n-propanol/water/25% ammonia.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

エラスチンの代表的ピリジニウム環含有架橋アミノ酸であるデスモシンおよびイソデスモシンと共に,最近構造が明らかにされたネオデスモシンおよびアロデスモシンを価値の低い牛の項靭帯,大動脈および肺から分離調製する方法について検討した.
加水分解物から架橋アミノ酸を分離する過程において,活性炭カラムを用いることにより,架橋アミノ酸の相互分離と共に,中性アミノ酸の大部分を除くことができた.活性炭カラムにより除去できなかった疎水性アミノ酸は,大口径シリカゲルカラムにより除去できた.デスモシンおよびイソデスモシンは,シリカゲルの分取カラムによる,アンモニア系の溶媒を用いた高速液体クロマトグラフィー(HPLC)により相互に分離し,それぞれ65%以上の収率で調製された.活性炭カラムで分画されたネオデスモシンおよびアロデスモシンは,酢酸系の溶媒を用いたセルロースカラムクロマトグラフィーおよびシリカゲルの分取カラムを用いたHPLCにより,精製することができた.

責任著者(Corresponding author)

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