In Vitro Assessment of the Effect of Interleukin-1β on Angiogenic Potential of Bone Marrow Cells

Abstract

Background Therapeutic angiogenesis for ischemic diseases has been successfully induced by the implantation of autologous bone marrow cells (BMCs). It is understood that interleukin (IL)-1β increases remarkably in ischemic tissue and has particular effects on angiogenesis. Thus, it is important to clarify how IL-1β would effect BMCs survival and angiogenic potential. Methods and Results The effect of IL-1β on BMCs survival, adhesion, and endothelial differentiation, as well as the production of angiogenic growth factors, was investigated using an in vitro assessment approach. BMCs were harvested from Zucker obese rats and cultured at a density of 3×10⁶ cells/ml with 5 ng/ml IL-1 β (IL-1β group) or without IL-1 β (control group). Survival and adhesion of BMCs were significantly increased in the IL-1β group than in the control group after 1, 3, and 7 days of culture (p<0.01). The release of vascular endothelial growth factor in supernatant was also significantly higher in the IL-1β group than in the control group after 3 and 7 days of culture (p<0.01). Furthermore, the number of differentiated endothelial cells derived from BMCs was significantly higher in the IL-1β group than in the control group after 7 days of culture (p<0.01). These results suggest that IL-1β has a positive effect on the angiogenic potential of BMCs in vitro. (Circ J 2006; 70: 1195 - 1199)