Circulation Journal
Online ISSN : 1347-4820
Print ISSN : 1346-9843
Vascular Medicine
Effects of H2S on Myogenic Responses in Rat Cerebral Arterioles
Lei LiuHong LiuDong SunWeili QiaoYoujian QiHong SunChangdong Yan
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2012 Volume 76 Issue 4 Pages 1012-1019

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Abstract

Background: The potential biological significance of hydrogen sulfide (H2S) has attracted growing interests in recent years, but its role in the myogenic response of rat cerebral arterioles has not been explored. Methods and Results: Rats were injected with NaHS (an H2S donor, 2-200μmol·kg-1·day-1, i.p.) or saline for 3 weeks. MBP was measured with a tail-cuff method. Cerebral arterioles were isolated and cannulated in an organ bath system, and vessel diameters were measured with an image-shearing device. Changes in diameter in response to stepwise increases in intravascular pressure (20-120mmHg) were investigated under no-flow conditions. After the treatments, plasma H2S increased and MBP decreased significantly. NaHS reduced the myogenic response in a dose-dependent manner. This effect was markedly attenuated by glibenclamide, a KATP channel blocker. Blockade of nitric oxide (NO) production with NG-nitro-L-arginine methyl ester (L-NAME, a NO synthase inhibitor) enhanced, whereas removal of the endothelium abolished the inhibitory role of NaHS on the myogenic response. Conclusions: For the first time it has been demonstrated that H2S decreases the myogenic response of cerebral arterioles in vivo, and this effect is endothelium-dependent and partially mediated by KATP channels. (Circ J 2012; 76: 1012-1019)

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© 2012 THE JAPANESE CIRCULATION SOCIETY
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