Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury

Chun-Ka Wong; Hayes Kam-Hei Luk; Wing-Hon Lai; Yee-Man Lau; Ricky Ruiqi Zhang; Antonio Cheuk-Pui Wong; George Chi-Shing Lo; Kwok-Hung Chan; Ivan Fan-Ngai Hung; Hung-Fat Tse; Patrick Chiu-Yat Woo; Susanna Kar-Pui Lau; Chung-Wah Siu

doi:10.1253/circj.CJ-20-0881

This article has now been updated. Please use the final version.

Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury

Chun-Ka Wong, Hayes Kam-Hei Luk, Wing-Hon Lai, Yee-Man Lau, Ricky Ruiqi Zhang, Antonio Cheuk-Pui Wong, George Chi-Shing Lo, Kwok-Hung Chan, Ivan Fan-Ngai Hung, Hung-Fat Tse, Patrick Chiu-Yat Woo, Susanna Kar-Pui Lau, Chung-Wah Siu

Author information

Chun-Ka Wong
Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Hayes Kam-Hei Luk
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Wing-Hon Lai
Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Yee-Man Lau
Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Ricky Ruiqi Zhang
Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Antonio Cheuk-Pui Wong
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
George Chi-Shing Lo
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Kwok-Hung Chan
Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Ivan Fan-Ngai Hung
Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Hung-Fat Tse
Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Patrick Chiu-Yat Woo
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Susanna Kar-Pui Lau
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]
Chung-Wah Siu
Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong [China]

Keywords: COVID-19, Myocarditis, SARS-CoV-2, Stem cells

JOURNAL OPEN ACCESS FULL-TEXT HTML Advance online publication

Article ID: CJ-20-0881

DOI https://doi.org/10.1253/circj.CJ-20-0881

The final version of this article is now available: Vol. 84 (2020), No. 11 pp. 2027-2031

Details

Abstract

Background:SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.

Methods and Results:Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated.

Conclusions:Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.

Corresponding author

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