Micro-determination of Cardiac Glycosides by Means of the Embryonic Heart of Japanese Killifish Oryzias Latipes (III) : ACTION OF CARDIAC GLYCOSIDERS UNDER THE INFLUENCES OF ETHANOL AND ESERINE

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Eggs treated with hyaluronidase and eserine (10⁹g/cc, 30min) were exposed at 30°C to the Ringer solution of digitoxigenin, lanatoside C, g-Strophanthin or digitoxin. Sensitivity to cardiac glycosides was more raised in the eserinized egg than in the non-eserinized, and 10^-10g/cc concentration could be detected, and 1/2 × 10^-9g/cc determined quantitatively, using 0.02 cc of sample solution (contained 1 or 0.1% of ethanol).