Acid Mucopolysaccharase Activity in Lysosome of Rat Kidney

Abstract

1. Acid mucopolysaccharase activity in lysosome of rat kidney was demonstrated using hyaluronic acid and chondroitin sulphate A as substrates. 2. The enzymes were released from lysosomal particles by treating with Triton X-100, as well as lysosomal β-glucuronidase. 3. The optimal pH for acid mucopolysaccharase of lysosome were pH 4.1 for chondroitin sulphate A and pH 5.0 for hyaluronic acid as substrate in acetate buffer, but in phosphate buffer pH 6.8 for chondroitin sulphate A and pH 6.l for hyaluronic acid.