不溶性ナリンギナーゼによるバセペイユ果皮中のナリンギン濃度の変化
1986 年 19 巻 3 号 p. 204-208
詳細
1986 年 19 巻 3 号 p. 204-208
Water-insoluble naringinase was prepared by binding 1% native naringinase solution to DEAE cellulose, and its specific activity was confirmed to be 96% of the native one.
To check the enzyme activity of this preparation, the same water-insoluble enzyme binded on diatomaceous earth was employed by the courtecy of Dr. Shimoda. When 0.1% of native and two kinds of water-insoluble naringinase were added to 0.02% naringin solution, the decrease ratios of the substrate were 69,72 (DEAE), and 75% (Diat.), respectively.
These water soluble and water-insoluble enzymes were also reacted to banpeiyu peel and, as the result, the highest decomposition of naringin was obtained by the water-insoluble enzyme binded on diatomaceous earth.
