Compositional Analysis of Copoly (DL-Lactic/Glycolic Acid) (PLGA) by Pyrolysis-Gas Chromatography/Mass Spectrometry Combined with One-step Thermally Assisted Hydrolysis and Methylation in the Presence of Tetramethylammonium Hydroxide

Abstract

Rapid and precise compositional analysis of copoly (DL-lactic/glycolic acid) (PLGA) was performed by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) combined with one-step hydrolysis and methylation in the presence of tetramethylammonium hydroxide (TMAH). Pyrolysis of PLGA with TMAH gave two characteristic products, derivatives of DL-lactic acid and glycolic acid, which directly reflect the average molar composition of PLGA. The analytical results for PLGA samples with various compositional ratios were in good agreement with those obtained by ¹H-NMR spectrometry, and the precision was satisfactory.