Biosynthetic Study of Amphidinolide W

抄録

The biosynthetic origins of amphidinolide W (1) were investigated on the basis of ¹³C-NMR data of ¹³C-enriched samples obtained by feeding experiments with [1-¹³C], [2-¹³C], and [1,2-¹³C₂] sodium acetate in cultures of a strain Y-42 of the dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from a hexaketide chain, two acetate units, four isolated C₁ units from C-2 of acetates, and four branched C₁ units from C-2 of acetates. The acetate-incorporation patterns for C-1–C-2–(C-21) and C-8–C-18–(C-23, C-24) of 1 corresponded well to those for C-1–C-2–(C-27) and C-5–C-15–(C-28, C-29) of amphidinolide H (2) isolated from this strain.