Synthesis and Triplex-Forming Ability of 2′,4′-BNAs Bearing Imidazoles as a Nucleobase

Yoshiyuki Hari; Satoshi Obika; Hiroyasu Inohara; Masahiro Ikejiri; Daisuke Une; Takeshi Imanishi

doi:10.1248/cpb.53.843

Notes

Synthesis and Triplex-Forming Ability of 2′,4′-BNAs Bearing Imidazoles as a Nucleobase

Yoshiyuki Hari, Satoshi Obika, Hiroyasu Inohara, Masahiro Ikejiri, Daisuke Une, Takeshi Imanishi

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Keywords: methylene bridged nucleic acid (BNA), locked nucleic acid (LNA), triplex, imidazole, oligonucleotide

JOURNAL FREE ACCESS

2005 Volume 53 Issue 7 Pages 843-846

DOI https://doi.org/10.1248/cpb.53.843

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Abstract

To expand the sequence of double-stranded DNA (dsDNA) targets in a triplex formation, 2′,4′-BNAs (2′-O,4′-C-methylene bridged nucleic acids) having imidazoles as a nucleobase were synthesized and incorporated into oligonucleotides. Triplex-forming ability of the modified oligonucleotides was evaluated by using melting temperature (T_m) measurements.

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