Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Regular Articles
Direct Determination of p-Hydroxymethamphetamine Glucuronide in Human Urine by High-Performance Liquid Chromatography
Rika NakajimaMuneo MorishitaSadakazu AisoTakehiko Yajima
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2006 Volume 54 Issue 4 Pages 493-495


High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak® Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.

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© 2006 The Pharmaceutical Society of Japan
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