2008 年 56 巻 8 号 p. 1168-1172
Repeated silica gel and octadecyl silica gel (ODS) column chromatography of the aerial parts of Artemisia princeps PAMPANINI (Sajabalssuk) led to the isolation of a new sesquiterpenoid, 3-((S)-2-methylbutyryloxy)-costu-1(10),4(5)-dien-12,6α-olide (2), along with two previously reported sesquiterpenoids: 8α-angeloyloxy-3β,4β-epoxy-6βH,7αH,8βH-guaia-1(10),11(13)-dien-12,6α-olide (1, carlaolide B) and 3β,4β-epoxy-8α-isobutyryloxy-6βH,7αH,8βH-guaia-1(10),11(13)-dien-12,6α-olide (3, carlaolide A). The structure of compound 2 was elucidated by spectroscopic data analysis, including one dimensional (1D) and two dimensional (2D) nuclear magnetic resonance (NMR) experiments. Of the isolates, compound 2 exhibited potent cytotoxicity against human cervix adenocarcinoma cells and induced apoptosis.