Synthesis, Characterization, Cytotoxic Activities, and DNA-Binding Studies of Ternary Copper(II) Complexes with New Coumarin Derivatives

Lei Jia; Xi-Ming Xu; Jun Xu; Long-Hai Chen; Peng Jiang; Fei-Xiang Cheng; Guang-Nong Lu; Qin Wang; Jin-Cai Wu; Ning Tang

doi:10.1248/cpb.58.1003

Regular Articles

Synthesis, Characterization, Cytotoxic Activities, and DNA-Binding Studies of Ternary Copper(II) Complexes with New Coumarin Derivatives

Lei Jia, Xi-Ming Xu, Jun Xu, Long-Hai Chen, Peng Jiang, Fei-Xiang Cheng, Guang-Nong Lu, Qin Wang, Jin-Cai Wu, Ning Tang

Author information

Lei Jia
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Department of Physics and Chemistry, Henan Polytechnic University
Xi-Ming Xu
School of Life Science, Lanzhou University
Jun Xu
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Long-Hai Chen
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Peng Jiang
School of Life Science, Lanzhou University
Fei-Xiang Cheng
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Guang-Nong Lu
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Qin Wang
School of Life Science, Lanzhou University
Jin-Cai Wu
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University
Ning Tang
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University

Keywords: copper(II) complex, coumarin derivative, DNA-binding, intercalative mode, cytotoxic activity

JOURNAL FREE ACCESS

2010 Volume 58 Issue 8 Pages 1003-1008

DOI https://doi.org/10.1248/cpb.58.1003

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Abstract

In this study, two novel complexes [Cu(MCVH)phen(H₂O)]·ClO₄ (1) and [Cu(MCLH)phen(H₂O)]·ClO₄ (2) (here, MCVH₂=(7-hydroxy-4-methyl-8-coumarinyl) valine, MCLH₂=(7-hydroxy-4-methyl-8-coumarinyl) leucine) have been synthesized and characterized by elemental analyses, molar conductance, infrared spectra (IR), ¹H-NMR and UV–Vis measurements. The interactions of them with calf thymus DNA (ct DNA) have been investigated by absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity measurements. Experimental results reveal an intercalative interaction with DNA for the complexes, furthermore the binding affinity of 2 is higher than that of 1 according to the calculated binding constant values. In addition, they were evaluated for their cytotoxic activities toward human prostate cancer cell (PC3), human liver cell (L02) and human myeloid leukemia cancer cell (HL-60) by acid phosphoatase assay. Both of them showed significant cytotoxic potency.

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