Determination of Schisandrin A and Schisandrin B in Traditional Chinese Medicine Preparation Huganpian Tablet by RP-HPLC

Abstract

A RP-HPLC method for separation and determination of Schisandrin A and Schisandrin B was presented, using a C18 Bondclone column, with methanol-water (V/V = 68:32) as mobile phase at a flow-rate of 1.00mL⋅min^-1, and UV detection at 220nm. The tested parameters included mobile phase composition and UV detection wavelength. Good linearities were observed within concentration ranges of Schisandrin A 0.008-4.8mg⋅L^-1 (r=0.9996), and Schisandrin B 0.005-3.1mg⋅L^-1 (r=0.9994), respectively. The limit of detection (LOD) (S/N=3) were 0.005mg⋅L^-1 Schisandrin A and 0.002mg⋅L^-1 Schisandrin B, respectively. The method was applied to determine the 2 compounds in a traditional Chinese medicine preparation for treatment of hepatic diseases, Huganpian Tablets. To eliminate matrix effect, Oasis HLB SPE extraction was used to purify the ultra-sonicately extracted solution of the drug sample. Combined with the HLB SPE purification procedure, the HPLC method gave satisfactory results for quantitation of Schisandrin A and Schisandrin B in 3 types of Huganpian tablet samples, with spiking recoveries ca 98% (RSD≤ 3.5%) (n=5).