Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363

This article has now been updated. Please use the final version.

Amide bond formation using 4-coumarate:CoA ligase from Arabidopsis thaliana
Takahiro Mori Kiyofumi WanibuchiHiroyuki MoritaIkuro Abe
Author information
JOURNAL FREE ACCESS Advance online publication

Article ID: c21-00404

Details
Abstract

Amide bond formation is one of the most fundamental reactions in organic chemistry, and amide bonds constitute the key functional groups in natural products, peptides, and pharmaceuticals. Here we demonstrate the chemoenzymatic syntheses of 4-coumaroyl- and hexanoyl-amino acids, using 4-coumarate: coenzyme A ligase from the model plant Arabidopsis thaliana (At4CL2). At4CL2 accepts 4-coumaric acid and hexanoic acid as the carboxylate substrates to generate acyl adenylates, which are captured by the amino group of amino acids to afford a series of N-acyl amides. This study shows the potential of 4CL for application as a biocatalyst to generate a series of biologically active amide compounds.

Content from these authors
© 2021 The Pharmaceutical Society of Japan
feedback
Top