Studies on Oxidized Starch Sulfates for Medical Purposes. V. Investigation on the Complex of Pepsin with Sulfates of Oxidized Starch and its Reduced Products.

Abstract

It was confirmed that the complex of pepsin with ROS-S and heparin is formed in an acid solution (pH 1.5∼4.4) and that the complex is dissociated at the pH above 5.2. The isolated complex has no proteolytic activity, but it was almost completely recovered by removing ROS-S or heparin as the barium salt or protamine complex. It was confirmed that the inhibitory action of polysaccharide sulfates on proteolytic action of pepsin is caused by the formation of inactive complex of pepsin with the sulfates.