Abstract
It was evidenced that action of the enzyme of Eremothecium ashbyii on 6, 7-dimethylribolumazine produced formaldehyde and formic acid, besides riboflavin, and 6-methyl-7-hydroxyriboumazine. The experimental data obtained support the mechanism suggested by Plaut for the formation of riboflavin. As for the formation of 6-methyl-7-hydroxyribolumazine, however, it has been proved that the compound was produced by oxidative rupture of the 7-methyl group of 6, 7-dimethylribolumazine, and this result gave an experimental ground to the assumption of Korte, et al.
