Studies of Nucleosides and Nucleotides. XXI. A New Synthesis of Thymidine 5'-Triphosphate and the Use of P¹, P²-Di-(2-cyanoethyl) pyrophosphate in the Nucleoside Triphosphate Synthesis.

Abstract

A new synthesis of thymidine 5'-triphosphate was achieved by the phosphorylation with P¹-diphenyl P²-morpholino pyrophosphorochloridate of 3'-O-acetylthymidine, followed by the reaction with pyrophosphate salt and alkaline removal of acetyl group. The reaction of 2', 3'-O-isopropylideneadenosine 5'-phosphoromorpholinochloridate with P¹, P²-di-(2-cyanoethyl) pyrophosphate was also investigated. After the alkaline removal of protecting groups only isopropylidene-ADP was obtained.