抄録
A new colorimetric determination of cholesterol was studied and the standard procedure was established. The principle was based on that cholesterol in acetone was oxidized to cholest-4-en-3, 6-dione by the Kiliani's reagent, and its p-nitrophenylhydrazone produced a blue purple color in alkaline solution of dimethylformamide. This color had an absorption maximum at 575 mμ and was very stable. It has been found that free cholesterol could be determined specifically even in the presence of esterified cholesterols, which gave no color under the standard procedure. The method has been applied to the analysis of free and total cholesterol in serum. Known amounts of cholesterol added to extract from serum were measured using the calibration curve by the standard procedure and the recoveries were quite satisfactory.
