Inhibition and Stimulation of the Biosynthesis of Protein and Nucleic Acid. I. Effect of Some Phenylalanine Analogs on the Aromatic Amino Acid Incorporation into Proteins of Ehrlich Mouse Ascites Tumor Cells in vitro

Abstract

4-NO₂-Phe and 4-F-Phe exert the inhibiting action to the Phe or Tyr incorporation into proteins of the intact Ehrlich mouse ascites tumor cells. The inhibiting effect of 4-F-Phe is similar in the intact and disrupted tumor cells and the intact liver cells, while the effect of 4-NO₂-Phe is different among these three materials ; little inhibition inthe disrupted tumor cells, and rather stimulation in the whole liver cells. Optimum incubation medium and conditions of these experiments were studied. Finally, the site of bilolgical systems, which is affected by amino acid analogs, is discussed.