Polynucleotides. I. Formation of Internucleotidic Linkage by Means of Dimethylformamide-Thionyl Chloride Complex

Abstract

Using a complex derived from thionyl chloride and dimethylformamide, a method of the formation of internucleotidic linkage was investigated. From 5'-O-tritylthymidine and 3'-O-acetylthymidine 5'-monophosphate, thymidinyl (3'-5') thymidine was obtained in the yield around 40∼50%.