Gas Chromatography of Catecholamines and Related Compounds

Abstract

A gas chromatographic method was described employing a 3.5% SE-30 siloxane polymer as the liquid phase which permitted the separation of the catecholamines and related compounds. The satisfactory separation of epinephrine, norepinephrine, dopamine, metanephrine and normetanephrine was achieved through the trimethyl-silylation with hexamethyldisilazane followed by the condensation with 2-pentanone.