Studies on Glutamic Oxalacetic Transaminase in the Rat. I. New Assay Procedure for Enzyme Activity in Crude Preparations

抄録

Although the effects of various agents on glutamic oxalacetic transaminase (GOT) in tissue have been studied, the presence of isoenzymes, which have different characters, showed the lack for quantitativity in many of assay methods and the possibility of misleading results. In this report, the assay procedure for this enzyme activity in liver homogenate of rats was modified respect to the existence of isoenzymes. One procedure is a modified manometric method consisting of a coupled-system with chemical decarboxylation of oxalacetate to eliminate the inhibitory action of mitochondrial enzyme (m-GOT), and the other is a new type of spectrophotometric procedure. The latter could also be employed for the approximate calculation of isoenzyme levels in homogenates in combination with electrophoretic procedures for isoenzymes ratio with cellulose acetate film.