Studies on Glutamic Oxalacetic Transaminase in the Rat. II. Selective Denaturation of Isoenzymes by Cellulose Acetate Treatment

Abstract

A new technique of mild denaturation of glutamic oxalacetic transaminase on the surface of cellulose acetate film was introduced as an application for an assay procedure of enzyme activity in crude materials. This treatment modified the enzymatic character of mitochondrial enzyme in the presence of phospholipids in crude preparations after which the isoenzymes were estimated quantitatively using a concentration of substrate adequate for the enzyme in the soluble fraction which was a minor component in rat liver.