Abstract
A rapid polarographic microdetermination of D-glucose with β-D-glucose oxidase in direct oxygen consumption mode is described. The apparatus composed of polarographic oxygen electrode, recorder (2 mV full scale), voltage prebox for increasing the sensitivity by cancelling unnecessary voltage of the output voltage of oxygen analyzer, and specially designed vial fixed on the magnetic stirrer. A trace amount of sodium azide was used to inhibit catalase contained in the biological sample and crude glucose oxidase. In this method, the relation between the amount of D-glucose and the amount of consumed oxygen gave a straight line at the range of 0.5 to 600 μg of D-glucose. The results obtained by this method on serum glucose with internal standard were enough consistent with those obtained by o-toluidine-borate method. The result on blood is obtainable within 5 min after a blood sample (5 μl) was collected from a patient.
