Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Gas Chromatographic Analysis of Disaccharides. I. Analysis of Disaccharides as Trimethylsilyl and Trifluoroacethyl Derivatives of Corresponding Alditols
HIROSHI NAKAMURAZENZO TAMURA
Author information
JOURNAL FREE ACCESS

1970 Volume 18 Issue 11 Pages 2314-2321

Details
Abstract
Disaccharides in a mixture are reduced to the corresponding disaccharide alditols with sodium borohydride and analysis is performed as trimethylsilyl or trifluoroacethyl derivatives by gas chromatography. Trifluoroacetylation of carbohydrates with trifluoroacetic anhydride is accomplished in N, N-dimethylformamide in a few minutes at room temperature. Quantitative analysis in microscale is achieved both by the extraction of trimethylsilyl derivatives with n-hexane from the reaction mixture and by the use of an electron capture detector sensitive to TFA derivatives. Determination limits of 0.5μg of disaccharide as TMS derivative and 0.1 μg as TFA derivative are successfully achieved.
Content from these authors
© The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top