Abstract
3, 3', 4'-Trihydroxyflavone combines with magnesium ion in N, N-dimethylformamide to form a strongly fluorescent chelate which is useful for the determination of micro amounts of magnesium. A linear relationship between the fluorescence intensity and concentration of magnesium was found in the range from 0 to 5.0 μg of magnesium. The chelate which shows excitation maximum at 450 mμ and a fluorescence maximum at 503 mμ has a metal to ligand ratio of 1 : 1.
