Analytical Chemical Studies on Amino Sugars. IV. Determination of 2-Deoxy-2-sulfamido-D-glucose and 2-Acetamido-2-deoxy-D-glucose in Heparin

Abstract

A novel spectrophotometric method for microdetermination of 2-deoxy-2-sulfamido-hexose and 2-acetamido-2-deoxyhexose residues in heparin is described. Application of MBTH method to heparin and acid hydrolysate of heparin gives sulfamidohexose content and total hexosamine content, respectively. The difference of the two values corresponds to acetamidohexose content of the mucopolysaccharide.