抄録
A trinucleoside diphosphate, GpUpA was synthesized using a dinucleotide 2', 3'-cyclic phosphate as an intermediate. The synthetic approach involved a condensation of N, 2', 5'-O-triacetylguanosine 3'-phosphate (I) and uridine 2'(3')-phosphate (II) to yield the dinucleotide with 2', 3'-cyclic phosphate (III) (Chart 1). Pancreatic RNase treatment of the product was followed by acetylation of the 2'-hydroxyl group of uridine to give the protected dinucleotide (VII). The overall yield from the mononucleotide was 41%. The unfractionated dinucleotide was used for the condensation with the protected nucleoside bearing the 5'-hydroxyl group (VIII). The isolated yield of the unprotected product was 12% from the dinucleotide.
