抄録
Procedures for the purification of acidic growth factors for Bifidobacterium bifidum from carrot were developed. These involved adequate solvent extraction and subsequent successive chromatography on Amberlite IR-45, IRC-50, IRA-68, and SE, DEAE, QAE Sephadex which included a proper precipitation procedure for the SE-Sephadex eluate, and preparative paper electrophoresis. Among five kinds of growth factors separated, the major growth factor and the most acidic factor were highly purified. Both factors differed from coenzyme A and its known precursors, while one of the weaker acidic factors coincided with D-pantetheine 4'-phosphate in chromatographic and electrophoretic behaviors.
