Abstract
Molecular weight of the minor bifidus factor (III) in carrot was 700-800. III was converted to 3'-dephospho-coenzyme A (DP-CoA) with KCN or 2-mercaptoethanol. It suggested that III was 3'-dephospho-coenzyme A-S-sulfonic acid (DP-CoASSO3H). This was confirmed by amino acid and phosphoric acid analysis and ultraviolet spectrum. Further synthesized DP-CoASSO3H coincided with III in behaviors of gel filtration, paper electrophoresis, thin-layer chromatography and ultraviolet spectrum. Coenzyme A-S-sulfonic acid (CoASSO3H) was also synthesized, and significances of these S-sulfonated compounds are discussed.
