Studies on the Metabolic Fate and the Pharmacokinetics of 5-n-Butyl-1-cyclohexyl-2, 4, 6-trioxoperhydropyrimidine (BCP) in Man. II. Determination of BCP and Its Metabolite, 1-Cyclohexyl-5-(3-hydroxybutyl)-2, 4, 6-trioxoperhydropyrimidine by Ultraviolet Absorption Method

Abstract

The determination-procedures of BCP and its urinary metabolites, 1-cyclohexyl-5-(3-hydroxybutyl)-2, 4, 6-trioxoperhydropyrimidine (II) and 5-n-butyl-1-(4-hydroxycyclohexyl)-2, 4, 6-trioxoperhydropyrimidine (IV) were investigated by ultraviolet absorption method. Total and free BCP in a urine were determined separately by changing the conditions for extraction. The metabolite II in a urine was determined selectively by converting it to 3-cyclohexyl-7-methyl-1, 2, 3, 4, 6, 7-hexahydro-5-H-pyrano (2, 3-d) pyrimidine-2, 4-dione (XV), though IV was not determined by this method as the separation from the unknown metabolites was not successful.