Simultaneous Determination of Ethanol and Carbon Dioxide by Two-Order Microdiffusion Analysis and Its Application to Analysis of Alcoholic Fermentation
1971 年 19 巻 5 号 p. 1041-1045
詳細
1971 年 19 巻 5 号 p. 1041-1045
A procedure and an apparatus of two-order microdiffusion analysis have been devised and applied to the simultaneous determination of ethanol and carbon dioxide. The conditions and the limitation of the assay method are as follows:
1. Amount of outer chamber solution; 3ml
2. pH of outer chamber solution; 1.3-8.7
3. Range of determination and value of coefficient of variation; ethanol, less than 23mg, 2% carbon dioxide, less than 14mg, less than 6%
4. Diffusion temperature; 30-37°5. Diffusion time; either more than 24hr at 37° or 30 hr at 30°
6. Rate of recovery; ethanol, 99%(pH 1.3), carbon dioxide 100%(pH 1.3)
The assay method was also applied to simultaneous determination of ethanol and carbon dioxide evolved in the process of alcoholic fermentation.
