Abstract
The method for condensation of plasma kinins with high selectivity in biological samples was presented using siliconised silica gel. The method may be applied for kinins in urine, plasma, amuniotic fluid and lymph. The condensed kinin fraction was free from inorganic salts, urea, active amines and major proteins in plasma, and was charged directly on column chromatographic separation in the next step. Some of the kinins in biological fluid was purified as a single fluorescen band by dansylation on thin-layer chromatography within the two steps. The recovery of bradykinin (1 to 100μg) was 70 to 90% during the procedure.
