Abstract
The novel partial hydrolysis of peptide bonds by using N→O acyl migrative reactions followed by the methanolysis of the resulting ester linkages were studied to give the successful application with tetrahydro derivative of viomycin, a tuberculostatic antibiotic. Also, the partial hydrolysis of perhydroacetylviomycin yielded two peptide fragments. The sequential analysis of these fragments by Edman-dansyl procedures and the end group analysis concluded that the primary structure of viomycin is formulated as XXV. The sequence is well compatible to the chemical formula I, the one of recently proposed two structures.
