Abstract
The callus derived from the petiole of Panax ginseng has been vigorously grown on Murashige and Skoog's agar medium (minus glycine) supplemented with 2, 4-dichlorophenoxyacetic acid. Saponins such as ginsenosides Rb1 (I) and Rg1 (II) were obtained via the column of Sephadex LH-20 and silica gel, respectively. Sapogenins, such as panaxadiol (III), panaxatriol (IV) and oleanolic acid (V), were also isolated in crystalline form from the hydrolysates of crude saponins. The callus contains a considerable amount of saponins and the thin-layer chromatograms of the saponins are similar to those of ginseng root saponins.
