Abstract
It was demonstrated that the precipitate at 105000×g of cell-free extracts of Cephalosporium caerulens exhibited the activities for cyclizing 2, 3-oxidosqualene into lanosterol and 3β-hydroxyprotosta-17 (20)[16, 21-cis], 24-diene. However, the cell-free preparations possessed no activity for cyclizing into 3β-hydroxyprotosta-13 (17), 24-diene. Major part of these activities were located in the microsomal fraction which precipitates at 105000×g and deoxycholate treatment solubilized these cyclases.
