Abstract
The quantitative determination methods of tolbutamide (TB) and its two metabolites, namely 1-butyl-3-p-hydroxymethylphenylsulfonylurea (HTB) and 1-butyl-3-p-carboxyphenylsulfonylurea (CTB), in blood and urine were developed, which had been required especially for the detailed pharmacokinetic studies of TB involving the administration of these metabolites. These compounds were satisfactorily separated by extracting from the bioligical fluids with organic solvents utilizing the differences of pKa values and distribution coefficients to organic solvents among these compounds. The quantitations were made spectrophotometrically at ultraviolet region for blood specimens and at visible region for urine specimens after the reaction with 2, 4-dinitrofluorobenzene. The results of recovery tests are also given for all the procedures established.
