Author's Organization：Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Chiba Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Chiba
Periodical change in the binding of carbon monoxide with P-450 reduced by NADPH was found to show diphasic pattern. Effect of concentration of oxygen in the atmosphere and effect of pretreatment of rats with phenobarbital and 3-methylcholanthrene on the first and second phases were investigated. Diphasic binding of carbon monoxide with NADPH-reduced P-450 suggested the existence of multiple P-450 species.
