Abstract
The L- and D-amino acids could be resolved by coupling N-trifluoroacetyl-prolyl chloride (L-TPC) as the resolving agent by gas chromatography. The reaction conditions were studied in detail, the solvents, the amount of resolving agent and reaction time for quantitative derivatization. The 22 stationary phases were studied for chromatographic separation. Relative retention times and separation factors were determined for each enantiomeric pair of 15 amino acids and glycine. Relation between separation factors and structure of amino acids were also discussed.
