1974 Volume 22 Issue 10 Pages 2413-2420
The fluorescence of dansyl amino acids and dansyl proteins in aqueous media was found to be greatly enhanced by addition of cycloheptaamylose. This finding was successfully applied for improvement of the assay procedure of amino acids and proteins. Dansyl amino acids of 50 picomoles per ml in final concentration was estimated. Interaction of cycloheptaamylose with dansyl amino acids was thermodynamically studied. The entropies and the enthalpies were found to vary with polarities of the dansyl amino acid.