Chemical Modification of Proteins. VI. The Rea tion of N-(p-(2-Benzimidazolyl)phenyl)maleimide (BIPM) with the Thiol Groups of Egg Albumin

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The reaction of N-(p-(2-benzimidazolyl) phenyl) maleimide (BIPM) (1) with egg albumin was examined and detailed chemical and spectroscopic analyses of the modified protein were performed. BIPM reacted specifically with thiol groups in egg albumin to give fluorescent adducts in agreement with the empirical rule previously proposed by the authors. The incorporated BIPM exists as a succinamic acid form 3 in the protein. These results are of significance in the use of BIPM as a fluorescent thiol reagent for proteins.