Studies on Peptides. XLIX. Synthesis of the Octadecapeptide corresponding to the Entire Amino Acid Sequence of Porcine β-Melanocyte-stimulating Hormone

Abstract

The octadecapeptide corresponding to the entire amino acid sequence of porcine β-MSH was synthesized by assembling four peptide subunits ; Z (OMe)-Asp (OBzl)-Glu-(OBzl)-Gly-OH (I), Z (OMe)-Pro-Tyr-Lys (Z)-Met-NHNH₂ (II), Z (OMe)-Glu (OBzl)-His-Phe-Arg (Tos)-Trp-Gly-OH (III) and Z (OMe)-Ser-Pro-Pro-Lys (Z)-Asp (OBzl)₂, followed by deblocking of all protecting groups with hydrogen fluoride. The synthetic porcine β-MSH was 3.16 times active than our synthetic human β-MSH (6.2×10⁹ MSH unit/g).