Abstract
The reaction mechanism of the fluorometric determination of dulcin with sodium nitrite was studied and estimated as follows : Dulcin produces 4-ethoxybenzenediazonium (4EBD) ion via. p-phenetidine by the action of nitrous acid. The ion itself decomposes in part to the substances possessing the active methylene neighbouring to a carbony group. Finally, 4EBD ion reacts with the active methylene in the presence of an alkaline solution containing nitrous oxide to form the essential fluorescent compound, 1, 3-bis (4-ethoxyphenyl)-5-tetrazolone.
