Abstract
A simple and sensitive method is described for separatory determination of prostaglandin E and prostaglandin F. This method is based on three principles : (1) Prostaglandin E and prostaglandin F are oxidized by prostaglandin dehydrogenase, resulting reduced nicotinamide-adenine dinucleotide (NADH). (2) The sensitive determination of reduced NAD is performed by enzymic cycling with resazurin which serves as the terminal electron acceptor. (3) Alkaline treatment converts only prostaglandin E to insensitive material to prostaglandin dehydrogenase. This method was capable for separatory determination of prostaglandin E and prostaglandin F in the order of 10-11 mole.
