Abstract
Two models have been proposed for the reaction between mercaptoundecahydrododecaborate ions and protein molecules. Both models assume two-step reactions, the first step being different between the two models ; random- and monogamous-pairing models. Measuring the equilibrium concentrations of protein-ligand complex for many combinations of the borate and protein concentrations, and applying the modified simplex method to the data obtained, one can find out a set of parameter values by which the experimental results are best explained. These parameters include the number of active sulfhydryl groups, α, the number of active disulfide groups, β, per intact protein molecule, and the equilibrium constants, K1 and K2, for the first and second steps.
