Preparation of Yeast Mannan Derivatives by Stearoylation and Phosphorylation
1976 年 24 巻 5 号 p. 1100-1103
詳細
1976 年 24 巻 5 号 p. 1100-1103
The mannan from baker's yeast was modified by stearoylation and phosphorylation, and the water-soluble part of the resultant product, stearoyl mannan phosphate (SMP), was resolved by gel-filtration with Sephadex G-75 into two fractions, SMP-Fr. I and -Fr. II. The former fraction was found to contain three components, fatty acid, carbohydrate, and phosphate. The SMP-Fr. I was then fractionated by DEAE-Sephadex chromatography to give four subfractions, SMP-Fr. I-a, -b, -c, and -d. Each fraction except Fr. I-a was found to contain fatty acid, carbohydrate, and phosphate, and to be homogeneous in ultracentrifugal analyses.
