A New Photometric Method for the Determination of Serum Glutamate Pyruvate Transaminase Activity using Pyruvate and Glutamate as Substrates

Abstract

A new photometric method is presented for the assay of serum glutamate pyruvate transaminase activity on the basis of the determination of 2-oxoglutaric acid produced in the enzyme reaction under its optimal conditions by means of the previously established method for selective determination of the acid with diazotized sulfamethizole. The method gives reliable results and is simply performed with a small amount of sample with a wide range of activity.