Abstract
In order to obtain the specific antiserum used for radioimmunoassay of testosterone a new hapten-carrier conjugate was prepared from 15α-hydroxytestosterone 15-hemisuccinate by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-testosterone antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the related steroids. The results indicated that specific antiserum which is capable of differentiating testosterone from 5α-dihydrotestosterone and androstenedione to a certain extent would be produced by antigen whose steroidal moiety is coupled ot a coupled to a protein through the position remote from the inherent functional groups.
